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BDNF mRNA expression in HVC is correlated to the number of songs produced by each bird. (A) A strong and significant correlation was found between BDNF mRNA expression in HVC and the number of songs sung by each bird during a 30-min period (n = 31, r = 0.88, regression analysis; P < 0.001, ANOVA test). (B) In situ hybridization autoradiography of two birds that sang 25 and 52 songs, respectively. The density of silver grains reflects the relative level of BDNF mRNA expression.
