Skip to main content
. 2009 Apr;133(4):405–419. doi: 10.1085/jgp.200810186

Figure 1.

Figure 1.

The effect of Mg PPi on WT-CFTR. (A) A recording of WT-CFTR channels in an excised inside-out membrane patch. The channels were activated with 2 mM ATP plus 25 U/ml PKA (not depicted). After phosphorylation-dependent activation, application and removal of ATP result in rapid current rise and decay, respectively. 3 min after washout of ATP, channels were opened by different concentrations of PPi plus Mg2+. 15 mM PPi supplemented with 15 mM MgPPi still seems unable to saturate the macroscopic current. (B) Macroscopic current decay after ATP withdrawal (left) and the ensemble current relaxation after washout of 15 mM MgPPi (right). Channels opened by MgPPi appear to close more slowly than those opened by ATP.