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. 2009 Apr;133(4):405–419. doi: 10.1085/jgp.200810186

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© 2009 Tsai et al.

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Figure 3. — MgPPi locks open the closed WT channels. (A) Steady-state macroscopic current activated by 2 mM ATP plus PKA was further increased by a subsequent addition of 10 mM MgPPi. Red line represents single-exponential fit of the current relaxation upon the removal of ATP and MgPPi. (B) MgPPi locks open closed channels shortly after the removal of ATP. Note a 20-s time lapse between the removal of ATP and the application of MgPPi. It should also be noted that the initial part of the current relaxation seems to be faster but was too small to be resolved. Red line marks single-exponential fit of the current decay upon the removal of MgPPi. (C) As the washout time was prolonged to 40 s, fewer channels were locked open by 10 mM MgPPi. Superimposed double-exponential fit (red line) yields τ₁ = 1.86 ± 0.34 s and τ₂ = 32.46 ± 7.41 s (n = 5).