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. 2009 Apr;133(4):405–419. doi: 10.1085/jgp.200810186

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© 2009 Tsai et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Time-dependent reopening of WT-CFTR by 10 mM MgPPi. Mg PPi was applied at different time points after ATP washout. The current amplitude of the slow component (red squares, left y axis) and the fast component (blue squares, right y axis) derived from double-exponential fitting were normalized to ATP current at t = 0. For t = 0 ∼20 s, the fast component was too small to be resolved. Fitting the data points (red curve) by a single-exponential function estimates the lifetime of the C₂state to be 27.4 s.