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. 2009 Mar 16;206(3):691–705. doi: 10.1084/jem.20081278

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© 2009 Beauvais et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Sdc1 and the α_vβ₃/α_vβ₅ integrins are coexpressed in normal and tumor vasculature. (A) En face staining of fixed mouse aorta for mSdc1 (mAb 281.2) and mouse α_v (AB1923), β₃ (AB1932), or β₅ (AB1926) integrin subunits. Endothelial cells are present as cords and are identified by mAb 390 (anti-PECAM-1/CD31) staining. Results are representative of at least three independent experiments. Bar, 50 µm. (B) Sdc1 expression in aortic explants. Staining with mSdc1-specific mAb (green) or control rat IgG and β₃ or β₅ integrin–specific IgG (red) or control rabbit IgG in microvessels induced by 30 ng/ml FGF-2 after 7 d from mouse aortic explants grown in type-I collagen gels. PECAM-1 staining of microvessels is also shown. Results are representative of triplicate wells and at least two independent experiments. Bar, 50 µm. (C) Expression of Sdc1 and integrins in tumor vasculature. PECAM-1, Sdc1, or α_v, β₃, or β₅ integrin subunits are stained in frozen sections of spontaneous mammary tumors arising in mice because of MMTV promoter–driven expression of ectopic Wnt-1 or ΔN89β-catenin (three mice each). Arrows point to identical structures in the PECAM and Sdc1 or integrin costained panels to orient the reader. Results are representative of at least three independent experiments. Bar, 50 µm.