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. 2009 Mar 16;206(3):561–576. doi: 10.1084/jem.20081886

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© 2009 Linterman et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Serum autoantibodies and renal pathology in Roquin^san/san Sap^−/− mice and reduction of the GC response in SAP-deficient Roquin^san/san mice is caused by B cell–extrinsic factors. (a, left) Representative staining of Hep-2 slides for detection of IgG ANA in the serum of 8-wk-old female Roquin^san/san and Roquin^san/san Sap^−/− mice (n = 5 per group). (right) Detection of IgG anti-dsDNA serum antibodies in 6-mo-old female Roquin^san/san and Roquin^san/san Sap^−/− mice determined by immunofluorescence staining using C. luciliae substrate. Data are representative of three independent experiments (n ≥ 5 per group). (b) Representative images of kidney sections stained with H&E (top) or viewed under an electron microscope (bottom) from 6-mo-old female Roquin^san/san Sap^−/− mice. Roquin^san/san Sap^−/− mice show slight mesangial expansion on H&E staining but no electron-dense deposits. Bars: (top) 100 µm; (bottom) 5 µm. (c) Score of nephritis severity in 6-mo-old female Roquin^+/+, Roquin^san/san, and Roquin^san/san Sap^−/− mice as determined by histological analysis according to the criteria given in Table S1 (available at http://www.jem.org/cgi/content/full/jem.20081886/DC1). Each symbol represents one mouse. Horizontal bars indicate medians. (d) Gating strategy for assessing the HEL-specific GC response (top) and graphic representation (bottom) of the total number of HEL-specific GC cells per spleen in mice with the indicated genotypes 7 d after cotransfer of SW_HEL B cells and HEL^2x-conjugated SRBCs. Data are representative of three independent experiments (n ≥ 4). Each symbol represents one mouse. (e) Gating strategy for determining HEL-specific extrafollicular plasma cells (top) and graphical representation (bottom) of the number of HEL-specific plasma cells 5 d after cotransfer into mice with the indicated genotypes. Data are representative of two independent experiments (n ≥ 3 per group). p-values are indicated on graphs.