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. 2009 Mar 16;206(3):681–689. doi: 10.1084/jem.20082100

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© 2009 Hikida et al.

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Figure 2. — The frequency of NP-specific IgG1⁺ GC B cells and memory B cells is diminished in PLC G1KO mice. (A) Representative data depicting analysis of the frequency of GC B and memory B cells. Spleen cells were stained as described in Materials and methods and 2.5 × 10⁶ cells were analyzed in each panel. The numbers of GC and memory cells per 2.5 × 10⁶ cells are indicated. (B) The frequency of NIP binding IgG1⁺ memory and GC B cells was examined on days 10 and 40 after the primary immunization. The frequency per 10⁶ cells is indicated. Each group consisted of at least five mice, and representative data from three repeated experiments are shown. Error bars indicate the SD for each group. **, P < 0.01; *, P < 0.05.