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. 2009 Mar 23;184(6):881–894. doi: 10.1083/jcb.200808041

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© 2009 Eastman et al.

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Figure 5. — SPG20 interacts with TIP47. (A) Directed yeast two-hybrid analysis of interactions between Gal4-SPG20 and candidate LD-associated proteins (Cav1, Cav3, ADRP, Rab18, TIP47, and Arf-1) fused to the VP16 activation domain. WWP1 is included as a positive control. (B) GST coprecipitation of YFP-SPG20 and TIP47-GST. Unfractionated 293T cell lysates and proteins bound to glutathione-agarose were detected by Western blotting with α-GFP and α-GST antibodies. WB, Western blot. (C) Yeast two-hybrid analysis of interactions between SPG20 and TIP47. The DNA-binding constructs containing full-length SPG20, SPG20dPY, SPG20dMIT, and SPG20-1110mt were tested against the VP16 activation domain fused to WWP1 or TIP47. Gal4-Rab9 was included in the experiment as a control for TIP47 binding. (D) Colocalization of SPG20 and TIP47. Endogenous TIP47 is stained with an α-TIP47 monoclonal antibody in HeLa cells stably expressing either YFP-SPG20 (top) or unfused YFP (bottom). Error bars represent the standard deviation of the mean. Bars, 10 µm.