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. Author manuscript; available in PMC: 2009 Jun 19.
Published in final edited form as: Nat Cell Biol. 2007 May 7;9(6):636–645. doi: 10.1038/ncb1590

Figure 7.

Figure 7

TRPC7 is not regulated by STIM1. (a, b) HEK cells were transfected with low (a) or high (four times more cDNA; b) levels of TRPC7 alone or with ΔERM–STIM1D76A and stimulated with carbachol. The mode of TRPC7 function was determined by measuring the sensitivity to inhibition by 1 and 10 μM Gd3+. Typical I/V relationships at the indicated conditions and the mean ± s.e.m. of four experiments with low and five experiments with high levels of SLC26A7 are also shown. (c) TRPC7 was transfected into HEK cells treated with scrambled (control) or STIM1 siRNA and the effect of STIM1 knockdown on TRPC7 current and its sensitivity to Gd3+ were measured. (d) TRPC7 was expressed alone or with the constitutively active STIM1D76A and the spontaneous, carbachol-stimulated and total current were measured. STIM1D76A did not increase spontaneous activity of TRPC7. The error bars in c and d indicate the mean ± s.e.m. of four experiments.