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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Neurobiol Dis. 2009 Apr 15;35(1):3–13. doi: 10.1016/j.nbd.2009.04.002

Figure 5. Effects of maternal and offspring diet on Iba-1 expression in rat brain.

Figure 5

Male rats were segregated into CD/CD, CD/HFD, HFD/CD and HFD/HFD groups as described in Methods, and the effects of maternal and offspring diet on expression of the microglia marker Iba-1 was evaluated by immunocytochemistry and Western blot as described in Methods. (A) The levels of Iba-1 protein measured by Western blot. Data are means and SEM, with 9-10 individual rats per group, and ANOVA indicated an overall significant effect of Offspring diet on Iba-1 expression in the cortex (text insert). (B) Representative low-magnification and high-magnification (insert) images depict the pattern of Iba-1 staining in cortex as observed in sections taken at the level of the lateral ventricle.