Figure 2.

Calsenilin (CLSN) reverses the potentiation of calcium signaling conferred by wt and mutant PS1. Ins(1,4,5)P₃-mediated calcium signaling in oocytes injected with water or CLSN alone (A), wt PS1 or wt PS1 plus CLSN (B), and PS1_M146V or PS1_M146V plus CLSN (C). (a) Representative families of calcium signals evoked by flash durations of 20, 45, and 90 ms in single oocytes. (b) Mean peak fluorescent intensity as a function of flash duration (n = 10–12 per condition). Note that the responses of control cells are shown for comparison (light solid lines) in Bb and Cb. (c) Mean responses after supramaximal Ins(1,4,5)P₃ stimulation (n = 27–32 per condition; *, P < 0.001).