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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Dev Biol. 2009 Mar 21;330(1):54–72. doi: 10.1016/j.ydbio.2009.03.009

Figure 8.

Figure 8

RALDH2 expression is reduced in chick LS segments with ectopic expression of Hoxd11. A-F. Normal patterns of RALDH2 expression among MNs (Isl1(2)+ cells) within LS2, LS4, and LS6 sections at stage 24 (A-C) and stage 29 (D-F). G-I. RALDH2 and Isl1(2) expression at stage 24 in motor column sections from normal, β-actin∷d10 and β-actin∷d11 embryos. Despite a substantial population of Isl1(2)+ MNs, the Hoxd11-transfected motor column shows reduced RALDH2 expression (I). J-K. RALDH2 expression in sections from stage 29 β-actin∷d10 and ß-actin∷d11 embryos. RALDH2 expression is prominent in lateral EGFP+ cells in the Hoxd10-transfected section. In contrast, there is no overlap in RALDH2 and EGFP expression following Hoxd11 transfection. L. Plots of % change in fluorescence intensity on transfected and non-transfected sides of anti-RALDH2 stained sections. Average fluorescence intensity was determined for circumscribed motor areas to correct for any differences in motor column size on transfected and non-transfected sides. Each dot=1 section, 0=no difference between transfected and non-transfected sides, negative number=decrease on transfected side. Red bars=mean % change in fluorescence for embryos transfected with the same Hox construct and sacrificed at the same stage. (Numbers and paired t-test p values in text) Scale bar=100 μm.