Figure 4.
PE decreases CA1 epileptiform bursts in one in vitro epilepsy model. Field recordings were made from CA1 stratum pyramidale in either zero Mg2+ aCSF or aCSF containing 100 μM PTX. (A) Representative traces of population spikes recorded from CA1 stratum pyramidale in zero Mg2+ aCSF before, during, and after a 50 μM PE challenge. (B) In zero Mg2+ aCSF, 50 μM PE produced a significant decrease in population spike frequency (70±5% of baseline), an effect that was blocked by pre-treatment of slices with 100 nM of 5-MU (96±0.3%). (C) In aCSF containing 100 μM PTX, 50 μM PE provided a minimal but not significant decrease in population spike frequency (93±2% of baseline). For each experiment n=8.