Table II.

Utp6 mutations isolated in the screen for CS

Strain	Mutations	Conservation	Location
cs-1	I88T	I in 61% of Utp6	HAT1, position 2
	Y96C	Y in HATs	HAT2, position 10
	N191D	None	HAT3, position 33
	Q211Z	None	C-term
cs-2	S84P	S in 39% of Utp6	N-term
	G99E	A/G in all Utp6	HAT1, position 13
cs-3	R165G	R in HATs	HAT3, position 7
	E218D	E in 52% of HATs	C-term
cs-6	I12N	I in 45% of Utp6	N-term
	I146N	I in 68% of HATs	HAT2, position 23
	D177N	E/D in 65% of Utp6	HAT2, position 19
	S438R	None	C-term
cs-9	K34E	K in 65% of Utp6	N-term
	H289R	None	C-term
cs-10	L136P	L in 84% of Utp6	HAT2, position 16
	K195R	K in 84% of Utp6	C-term
	S236P	None	C-term
cs-11	L139S	L in 29% of Utp6	HAT2, position 6
	F192L	None	C-term
cs-12	Frameshift 238	None	C-term
cs-14	E210V	E in 26% of Utp6	C-term
	N217D	None	C-term
	K290R	None	C-term
	I381T	None	C-term
cs-16	Q219Z	None	C-term
cs-17	T100A	T in 55% of Utp6	HAT1, position 14
	N175S	N in 23% of Utp6	HAT3, position 17
	Frameshift 232	None	C-term
cs-21	S87T	S in 42% of Utp6	HAT1, position 1
	K138E	None	HAT2, position 15
	N336S	None	C-term
	K389E	K in 26% of Utp6	C-term
cs-22	L136P	L in 35% of Utp6	HAT2, position 13
	K187R	K/R in 87% of Utp6	HAT3, position 29

Thirteen strains were isolated in the screen for mutations in Utp6 that confer CS (cs-1 through cs-22). Sequencing of the utp6 gene from each strain revealed the mutations listed. For each mutated amino acid, its conservation in HATs or in Utp6 homologs is listed, if any. Also for each mutated amino acid, its position in Utp6 is listed: N-term indicates the residue lies N-terminal to the HAT domain, within amino acids 1–86; C-term indicates the residue lies C-terminal to the HAT domain, within amino acids 192–440; residues within the HAT domain are noted by their position within each HAT motif. This table was revised from Champion et al. (2008) based on the new Utp6 alignment.