Table 1.
Conditioning Phase - Group Designation | Group N | CTA Conditioning | Extinction Phase – Group Designation | Group N | Extinction Training | 30-day Latency before SR test | SR Group N | SR Test Solution | ||
---|---|---|---|---|---|---|---|---|---|---|
Days 1, 3, 5 | Days 2, 4, 6 | Odd Days | Even Days | |||||||
CTA | 23 | SAC3 + LiCl4 | H2O | CTA+EU- EXT 1 | 11 | SAC | H2O+LiCl | H2O | 5 | SAC |
CTA+CSO- EXT 2 | 12 | SAC | H2O+ SAL5 | H2O | 6 | SAC | ||||
NO CTA | 24 | SAC | H2O + LiCl | NO CTA+ “EU-EXT” | 12 | SAC | H2O+LiCl | H2O | 6 | SAC |
NO CTA+ “CSO- EXT” | 12 | SAC | H2O+ SAL | H2O | 6 | SAC |
CTA-EU-EXT = “Explicitly Unpaired” extinction procedure in which both the CS and US are presented on alternate days
CTA-CSO-EXT = “Conditioned Stimulus Only” extinction procedure in which only the CS is presented every other day
SAC = saccharin salt dissolved in DI H2O (0.3% w/v; p.o.)
LiCl = lithium chloride injection dissolved in sterile, physiological saline (81mg/ml; 81mg/kg; i.p.)
SAL= sterile, physiological saline (0.9% w/v, NaCl; 1ml/kg; i.p.)