Figure 4.

GABARAP immunoreactivity in embryonic day 19.5 spinal cord sections derived from wild-type (+/+) and gephyrin-knockout (−/−) mice. Anti-GABARAP stains punctate structures in spinal cells of wild-type tissue; in addition, a diffuse staining is seen (A). Gephyrin appears in synaptic clusters of wild-type tissue (B, and see ref. 14). In tissue sections derived from gephyrin-deficient mice, GABARAP immunoreactivity is unaltered (C), whereas gephyrin immunoreactivity is lost (D). (Scale bar, 10 μm.)