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Yeast two-hybrid analysis of protein–protein interactions
| Protein tested | GABARAP | p16 |
|---|---|---|
| GABAARγ1/350–444 | + | − |
| GABAARγ2/351–445 | + | − |
| GABAARγ3/333–446 | − | − |
| GABAARδ/327–429 | − | − |
| Gephyrin, full-length | − | − |
| Gephyrin/2–152 | − | − |
| Gephyrin/153–348 | + | − |
| Gephyrin/349–736 | − | − |
GABARAP and p16 bait constructs were used in binary interaction assays with GABAAR subunit and gephyrin sequences. Positive interactions revealed by lacZ and LEU2 expression are marked with a +, whereas − denotes white cells.
