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. 2009 Jul;57(7):649–663. doi: 10.1369/jhc.2009.952812

Figure 4.

Figure 4

Assessment of concordance between visual and computer-assisted analyses of Bcl-2 immunostaining. TMAs containing tumor cores were stained for Bcl-2 using the diaminobenzidine-based detection method and counterstained with hematoxylin. Correlations were determined with 95% concordance level between (A) automatically vs manually determined immunopercentage of Bcl-2–positive cells (r = 0.8665; p<0.0001), (B) Bcl-2 autoscore vs Bcl-2 manual immunoscores (IS) (r = 0.8453; p<0.0001), and (C) Bcl-2 autoscore vs optical density (OD) × percentage of Bcl-2–positive cells as determined by algorithm (r = 0.9877; p<0.0001). The Kaplan-Meier method was applied to generate the survival curves, and univariate survival distributions were compared by the log-rank test (D–H). Manual (D) (p=0.04) and automated (E) (p=0.1) Bcl-2 immunopercentage, Bcl-2 manual IS (F) (p=0.04), and autoscore (G) (p=0.1), as well as OD × automated Bcl-2 immunopercentage (H) (p=0.1) were compared with overall survival for colorectal cancer patients. Continuous line depicts high and dashed line low Bcl-2 protein levels.