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. 2009 Jul;57(7):687–699. doi: 10.1369/jhc.2009.953208

Figure 6.

Figure 6

Figure 6

RARα proteins in activated HSCs do not colocalize with mitochondria, endosomes, or lysosomes. Mitochondria (A), endosomes (E), lysosomes (I), and endoplasmic reticulum (ER) (M) were visualized by using MitoTracker Deep Red 633, Organelle Lights Endosomes-GFP, LysoTracker Red DND-99, or Organelle Lights Lysosomes-RFP and ER-Tracker Red, respectively, and are shown in green. The cells were fixed and permeabilized (B,F,J), followed by staining with an anti-RARα antibody and appropriately labeled secondary antibodies, and are shown in red (C,G,K). Merged images (D,H,L) are also shown. Bar = 10 μm and applies to all the panels.