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Solubility of RARα proteins in activated HSCs. Activated HSCs were collected and sequentially solubilized by non-ionic (NP-40), mild ionic (sodium deoxycholate), and strong ionic (SDS) detergents (A). Each lysate, along with the residual pellet derived from the same amount of cells, was separated by SDS-PAGE and analyzed by Western blotting for RARα protein quantification (B). All protein bands after SDS-PAGE are shown by Coomassie Brilliant Blue R-250 staining (C).
