Figure 6.

Downstream signalling induced by reticular Bim or Puma and ER stress in the Bak–cb5-expressing clones. (A) BimΔTM– and Puma–cb5 do not activate XBP1. MEFs were either transduced or treated with tunicamycin (TN, 10 μg/ml) and lysed at the shown times for western blotting. (B) TRAF2-DN inhibits JNK phosphorylation promoted by reticular Bim and Puma. Cells were pretransduced twice with retroviruses expressing HA-TRAF2-DN (HA-TF2-DN), transduced 24 h later with the BH3-only molecules and lysed 17 h later for western blotting. (C) Depletion of IRE1α reduces JNK phosphorylation induced by BimΔTM– and Puma–cb5. Cells were transfected with the indicated siRNAs, transduced 36 h later with retroviruses expressing BimΔTM– and Puma–cb5, and lysed for western blotting 13 h later.