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. 2009 Jun 19;106(27):11358–11363. doi: 10.1073/pnas.0904998106

Fig. 1.

Fig. 1.

Time course of phospho-TrkA(pY490) (pTrkA) and full-length TrkA (TrkA) protein levels in hippocampal neurons exposed to NGF (+NGF) or deprived of NGF in a time ranging from 30 min to 24 h (-NGF). The amount of pTrkA due to 24 h of NGF deprivation is compared with that detectable after exposure to anti-Abeta antibody (4G8) (A) or to secretase inhibitors (γ and β) (B). Densitometric analyses of the corresponding experiments are reported in the Tables a and b. Values from phosphorylated TrkA were normalized with respect to full-length TrkA levels and were expressed as percentage of +NGF samples. *, P < 0.05 vs. +NGF; †, P < 0.05 vs. -NGF values (24 h). The identification of the 140-kDa Trk (pY490) species was obtained by immunoprecipitation of TrkA with a specific anti-TrkA antibody (see Methods) followed by immunoblotting with TrkA(pY490) antibodies before NGF exposure.