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. 2009 Jun 19;106(27):11358–11363. doi: 10.1073/pnas.0904998106

Fig. 3.

Fig. 3.

(A) Western blot analysis of AKT (pAKT) and phospholipase C γ (pPLCγ) phosphorylation levels from cultures before (Ctrl) and after 48 h of NGF (+NGF) exposure or deprivation of NGF (-NGF) in a time ranging from 30 min to 24 h. The Table at bottom reports the corresponding densitometric analysis. Values from pAKT were normalized using full-length AKT (AKT), whereas pPLCγ values were normalized using α-tubulin as internal control. All data were expressed as percentage of +NGF samples and are representative of 4 different experiments. *, P < 0.05 vs. +NGF values; †, P < 0.05 vs. 30 min of NGF removal values. (B) Western blot of PLCγ of neurons preincubated with scramble RNAi or silenced for p75, TrkA, or with both together after 24 h of NGF removal. All data were expressed as percentage of -NGF scramble samples and are representative of 4 different experiments. *, P < 0.05 vs. -NGF scramble values.