Table 1.

Extent of intact and condensed nuclei in NGF-deprived neurons (24 h) after exposure to TrkA inhibitors K-252a and CEP-2563

Variable	Intact nuclei	Condensed nuclei
+NGF	100	2.0 ± 0.7
-NGF	50 ± 3*	4.5 ± 0.9*
K-252a	80 ± 2	3 ± 0.4
CEP	78 ± 5	3 ± 0.8
K-252a/-NGF	83 ± 7†	2.5 ± 0.5†
CEP/-NGF	81 ± 4†	2.0 ± 0.8†

Two hours after incubation with K-252a (100 nM) and CEP-2563 (CEP) (200 nM), cells were washed 3 times and re-exposed for 24 h to a fresh medium with (K-252a; CEP) or without NGF (K-252a/-NGF; CEP/-NGF). The corresponding Western blot analysis showing the effect of TrkA inhibitors on TrkA(pY490) levels is reported in Fig. S2 a and b. Parallel experiments were performed with 10 μM Abeta 1-42 peptides in the presence or absence of K-252a and CEP-2563 (K-252a/Ab; CEP/Ab) (Fig. S2c and Table). Condensed nuclei are expressed as the mean ± SE of 10 fields of duplicate determinations of 5 independent experiments. Intact nuclei are reported as percentage of controls (+NGF).

*, P < 0.05 vs. cell survival of +NGF samples.

†, P < 0.05 vs. cell survival of -NGF or Abeta samples.