Skip to main content
. 2009 Jun 18;28(14):2114–2127. doi: 10.1038/emboj.2009.163

Figure 5.

Figure 5

ATP differentially regulates actin polymerization during macrophage polarity gradient. (A) Deconvolved images of F-actin cytoskeleton labelled with Texas Red-phalloidin in peritoneal macrophages isolated from P2X7R+/+ or P2X7R−/− mice after differentiation to M1 or to intermediate state 3 polarization state, unstimulated or stimulated for 5 min with ATP (5 mM), MTX (0.2 nM) or a combination of ATP and MTX, in the presence or absence of A740003 (10 μM, 5 min before ATP). Bar, 5 μm. Images are representative of three independent experiments. (B, C) M1 polarized peritoneal macrophages isolated from P2X7R−/− mice were treated with cytochalasin B (CB; 2.5 μg/ml, 5 min before MTX) and stimulated for 15 min with MTX (0.2 nM) or a combination of MTX and ATP (5 mM) or PPi (3 mM), and IL-1β release was detected by immunoblots (B) or by ELISA (C); n=3 independent cultures for ELISA and representative of two independent experiments, blots are representative of two independent experiments.