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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Cancer Res. 2009 May 19;69(11):4621–4628. doi: 10.1158/0008-5472.CAN-08-3402

Figure 3.

Figure 3

Effects of SFRP2 on cellular processes involved in angiogenesis: (a) SFRP2 increases endothelial cell migration in a wound scratch assay. The rate of wound closure is shown for controls and increasing concentrations of mouse recombinant SFRP2. The values are the means ± SEM, n=3 for each concentration. SFRP2 increased MEC cell migration at low concentrations ranging from 0.7pM–700pM. (b) SFRP2 inhibits hypoxia induced apoptosis in HCAEC cells. HCAEC’s were incubated in a hypoxic chamber for 24 hours with and without the addition of SFRP2. Relative Caspases 3 activity was measured as described in “Material and Methods” Section. SFRP2 inhibited hypoxic induced apoptosis at 70pM and 700pM (*p<0.02). (c) Mouse endothelial cell tube formation assay. SFRP2 induces endothelial tube formation at 8 hours in a concentration-dependent manner. (d) Tacrolimus inhibits SFRP2 induced tube formation in mouse endothelial cells.