Figure 5.

Effect of GM-Fc on Ba/F3 cells expressing the deletion and truncation GMRα isoforms. (A) The binding capacity of GM-Fc to Ba/F3 cells expressing the deletion and truncation GMRα from C57BL/6 mice was determined by FACS as indicated previously. (B) Ba/F3:pFB, Ba/F3:GMRα (C57BL/6), the deletion and truncation isoforms (C57BL/6) were cultured in the presence of either GM-CSF, GM-Fc, or IL-3-conditioned medium or in the absence of cytokine. Cell proliferation was determined using Alamarblue as indicated previously.