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(a) Southern blot of Streptomyces sp. TN 58 BamHI-digested total DNA hybridised with 32P-labeled pSET152 plasmid. Lanes 1–8, the eight studied exconjugants; lane 9, untransformed wild type Streptomyces sp. TN 58; lane 10, BamHI-digested pSET152 plasmid DNA (5.7 Kb); lane 11, the 1 kb ladder used as DNA marker. (b) Schematic representation of the integration of plasmid pSET152 in the chromosome of Streptomyces sp. TN 58 by site-specific recombination.
