Table 1.

Dielectric topography map of CcO^a

		Membrane center (Å)				Variation
Residue	Atom	190.0	194.0	197.0	200.0	Diel.	Width
Metal sites
CuA	(top)	0.09	0.06	0.05	0.04	±0.01	±0.01
	(bottom)	0.12	0.09	0.07	0.05	±0.01	±0.01
Heme a	Fe	0.53	0.44	0.38	0.32	±0.01	±0.01
Heme a3	Fe	0.52	0.43	0.37	0.31	±0.01	±0.02
CuB	Cu	0.51	0.42	0.35	0.29	±0.01	±0.02
BNCb		0.52	0.43	0.36	0.30	±0.01	±0.02
PLS candidatesa
His326	HD1	0.38	0.31	0.25	0.21	±0.02	±0.02
Heme a3	HA1	0.32	0.25	0.21	0.17	±0.01	±0.01
	HA2	0.30	0.23	0.19	0.15	±0.01	±0.01
	HD1	0.39	0.31	0.26	0.22	±0.01	±0.01
	HD2	0.36	0.29	0.24	0.19	±0.02	±0.01
Arg473	HE	0.34	0.27	0.22	0.18	±0.01	±0.01
Arg474	HE	0.29	0.23	0.19	0.15	±0.02	±0.01
Trp164	HE	0.46	0.38	0.32	0.27	±0.01	±0.01
Trp272	HE	0.34	0.26	0.21	0.17	±0.01	±0.01
PLS-related sites
Asp399	OD2	0.30	0.23	0.18	0.14	±0.01	±0.02
Waterc	O	0.36	0.29	0.24	0.19	±0.01	±0.02
K-channel residuesa
D-channel residuesa

The normalized potentials of the atoms shown have the meaning of the dielectric depth measured from the P-side of the membrane. The charge transfer between any of the atoms shown will produce membrane potential proportional to the difference of the potentials of the atoms. The shown data are discussed in the text.

^aThis is an abbreviated version of the data. A more complete table is given in the Supplementary information.

^bBNC is defined as the midpoint between Heme a₃/Fe and Cu_B/Cu.

^cThe water molecule located between the two propionates of heme a₃ and His326. Its position is estimated from the crystallography structure 1 V55[23].