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. Author manuscript; available in PMC: 2009 Jun 22.
Published in final edited form as: Mol Biochem Parasitol. 2007 Nov 22;158(1):32–45. doi: 10.1016/j.molbiopara.2007.11.009

Figure 2. A) Transcriptionally active PCR (TAP) first reaction.

Figure 2

94°C × 1 min then, 32 cycles of 94°C × 30 s, 60°C × 1 min and 68°C × 2 min, followed by 68°C × 10 min. Samples were run on a 1% agarose gel. For P. yoelii and P. falciparum gels, 1st and 2nd lanes for each construct represent reactions with plasmid and genomic DNA templates respectively. Constructs in P. yoelii by gel lane, 1&2: PyCSP 3&4: PyMSP1, 5&6: PyHEP17 (size difference due to amplification of exon with genomic template), 7&8: PySSP2, 9: positive control (GFP Template). Constructs in P. falciparum gel by lane, 1&2: PfCSP, 3&4: PfMSP1-42kD (42kD C-terminal region), 5&6: PfAMA1, 7&8: PfEBA175-RII (Region II), 9&10: PfExp1 (size difference due to amplification of exon with genomic template), 11&12: PfSSP2. 17X genomic for P. yoelii and 3D7 genomic for P. falciparum. B) Transcriptionally active PCR (TAP) second reaction: 94°C × 1 min then, 32 cycles of 94°C × 30 s, 60°C × 2 min and 68°C × 3 min, followed by 68°C × 10 min. Samples were run on a 1% agarose gel. For P. yoelii and P. falciparum gels, 1st and 2nd lanes for each construct are derived from dilutions of 1st TAP reactions used for template (plasmid and genomic origin, respectively). Constructs in P. yoelii gel by lane, 1&2: PyCSP, 3&4: PyMSP1, 5&6: PyHEP17 (size difference due to amplification of exon with genomic template), 7&8: PySSP2, 9&10: positive control (GFP Template) & negative control (no template). Constructs in P. falciparum gel by lane, 1&2: PfCSP, 3&4: PfMSP1-42kD, 5&6: PfAMA1, 7&8: PfEBA175-RII, 9&10: PfExp1 (size difference due to amplification of exon with genomic template), 11&12: PfSSP2.