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. Author manuscript; available in PMC: 2009 Jun 22.
Published in final edited form as: Mol Biochem Parasitol. 2007 Nov 22;158(1):32–45. doi: 10.1016/j.molbiopara.2007.11.009

Figure 5. T cell responses against PyHEP17.

Figure 5

BALB/c (PyHEP 17) were immunized IM with 50 μg plasmid DNA or TAP fragments encoding PyHEP17 3 times at 3-week intervals. Mice were sacrificed 2 wk after last immunization and splenocytes harvested. Recall T cell responses were assayed by (A) IFN-γ ELIspot (n=5/group), (B) Cytotoxic T lymphocyte (CTL) assays (n=5/group), (C) Intracellular Cytokine Staining (ICS) assays (n=3/group) or (D) Lymphoproliferation Assay (LPA) (n=5/group) using synthetic peptides representing defined CD8+ and/or CD4+ T cell epitopes on PyHEP17 (Table S1). CTL peptide pools: 15-mers (PyHEP61-75, PyHEP66-80, and PyHEP71-85); 9-mers (PyHEP61-69, PyHEP70-78, PyHEP76-84, and PyHEP84-92). Histograms represent the mean response for each group +/− standard deviation.