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. Author manuscript; available in PMC: 2009 Jun 22.
Published in final edited form as: Mol Biochem Parasitol. 2007 Nov 22;158(1):32–45. doi: 10.1016/j.molbiopara.2007.11.009

Figure 6. T cell responses against PyCSP.

Figure 6

BALB/c mice were immunized IM with 100 or 50 μg plasmid DNA or 50 μg of TAP fragments encoding PyCSP 3 times at 3-week intervals. Mice were sacrificed 2 wk after last immunization and splenocytes harvested. Recall T cell responses were assayed by Intracellular Cytokine Staining (ICS) (n=4/group) using synthetic peptides representing defined immunodominant or subdominant CD8+ or CD4+ T cell epitopes on PyCSP (Table S1). Histograms represent the mean response for each group +/− standard deviation.