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. Author manuscript; available in PMC: 2009 Jun 22.
Published in final edited form as: Mol Biochem Parasitol. 2007 Nov 22;158(1):32–45. doi: 10.1016/j.molbiopara.2007.11.009

Table 3.

Known Plasmodium spp. Antigen TAP PCR Reaction Details

Plasmodium spp. gene Size (bp)* Region Amplified Yield^
PyCSP 1173 Full length +
PyMSP1 1386 Full length ++
PyHEP17 665 Full length +++
PySSP2 2478 Full length +
PfCSP 1194 Full length ++
PfMSP1-42kD 1191 42kD C-terminal region +++
PfAMA1 1869 Full length +++
PfEBA175-RII 1851 Region II ++
PfEXP1 489 Full length +
PfSSP2 1728 Full length +/−
*

Size is for malaria antigen alone without promoter/terminator sequences.

^

For final product; Scale: −, +/−, +, ++, +++

Unless otherwise specified the following conditions were used: First Reaction: 94°C for 1 min, then 32 cycles of three-temperature PCR at 94°C for 30 sec, 60°C for 1 min, 68°C for 2 min, followed by a final extension time at 68°C for 10 min. Second Reaction: 94°C for 1 min, then 32 cycles of 94°C for 30 sec, 60°C for 2 min, 68°C for 3 min, followed by a final extension at 68°C for 10 min.