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. Author manuscript; available in PMC: 2009 Jun 22.
Published in final edited form as: Mol Biochem Parasitol. 2007 Nov 22;158(1):32–45. doi: 10.1016/j.molbiopara.2007.11.009

Table 4.

Known Plasmodium spp. Antigen Epi-TAP PCR Product Details

Plasmodium spp. gene Size (bp)* Region Amplified Yield^ Comments
PyCSP 1173 Full length +
PyMSP1 1386 Full length +++
PyHEP17 665 Full length +++
PySSP2 2478 Full length +
PfCSP 1194 Full length +
PfMSP1-42kD 1191 42kD C-terminal region +++
PfAMA1 1869 Full length +++ 1st reaction annealing done at 60°C for 1.5 min. and the final extension time done at 68°C for 3 min.
PfEBA175-RII 1851 Region II; FVO +++ 1st reaction annealing done at 54°C for 1.5 min. and the final extension time done at 68°C for 3 min.
PfEXP1 489 Full length ++
PfSSP2 1728 Full length +++ 1st reaction annealing done at 60°C for 1.5 min. and the final extension time done at 68°C for 3 min.
*

Size is for malaria antigen alone without promoter/terminator sequences

^

For final product; Scale: −, +/−, +, ++, +++

Unless otherwise specified the following conditions were used: First Reaction: 94°C for 3 min, then 32 cycles of three-temperature PCR at 94°C for 30 sec, 60°C for 1 min, 68°C for 3 min, followed by a final extension time at 68°C for 10 min. Second Reaction: 94°C for 1 min, then 32 cycles of 94°C for 30 sec, 60°C for 1.5 min, 68°C for 3 min, followed by a final extension at 68°C for 10 min.