Table 5.

Unknown Plasmodium falciparum Protein EpiTAP PCR Product Details

Plasmodium spp. gene	Size (bp)*	Region Amplified	Yield^	Comments
PFC0450w	327	Full length	++	1st reaction annealing done at 54°C for 45 sec.
PFC0700c	924	Full length	++
PFC0210c	1194	Full length	++	1st reaction annealing done at 60°C for 1.5 min. 2nd reaction ramp up done at 94°C for 3 min.
PF14_0074	1185	Full length	++
PF14_0751	564	Full length	++	1st reaction annealing done at 54°C for 45 sec.
PFL0800c	624	Full length	+++	1st reaction annealing done at 54°C for 1.5 min.
PFI0165c	7212	Full length	+++	1st Reaction: 92°C for 2 min, then 35 cycles at 92°C for 10 sec, 40°C for 30sec, 60°C for 12min, followed by a final extension time at 60°C for 10 min.
PFA0515w	4413	Full length	++
PFD0425w	2952	Single exon	++	1st Reaction: 92°C for 2 min, then 35 cycles at 92°C for 10 sec, 45°C for 30sec, 60°C for 12min, followed by a final extension time at 60°C for 10 min.

^*Size is for malaria antigen alone without promoter/terminator sequences

^{^}For final product; Scale: −, +/−, +, ++, +++

Unless otherwise specified the following conditions were used: First Reaction: 94°C for 3 min, then 32 cycles of three-temperature PCR at 94°C for 30 sec, 60°C for 1 min, 68°C for 3 min, followed by a final extension time at 68°C for 10 min. Second Reaction: 94°C for 1 min, then 32 cycles of 94°C for 30 sec, 60°C for 1.5 min, 68°C for 3 min, followed by a final extension at 68°C for 10 min.