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. 2009 Jul 1;4(7):e6124. doi: 10.1371/journal.pone.0006124

Figure 5. P38MAPK is not implicated in the response to IM.

Figure 5

(A) Sensitive and resistant K562 and Lama84 cell lines were treated with indicated concentrations of IM for 1 and 2 hours, lysed and analyzed by immunoblotting against active and total p38MAPK by using 50 µg of total cell lysates. As a loading control membranes were re-probed against tubulin (data not shown). (B) K562-S and K562-R were incubated with the indicated amount of IM in the presence (white bars) or absence (black bars) of SB203580 and then viability was determined 48 hours later. The histogram represents at least 3 independent experiments performed in triplicate cultures. Bars: mean±Standard Deviation. (C) Same as in B but using BIRB0796. Viability was referred to controls treated only with SB203580 or BIRB0796 (in the case of co-incubation with inhibitor) or vehicle (DMSO) in the case of IM alone. Treatment with SB 203580 or BIRB0796 alone induces toxicity lower than 2% in resistant and sensitive cell line.