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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Nat Immunol. 2008 Jun 22;9(8):927–936. doi: 10.1038/ni.1626

Figure 2. Ikzf1-/- pro-B cells can trans-differentiate into macrophages.

Figure 2

Pax5-/-, Rag2-/- and Ikzf1-/- pro-B cells were plated on S17 stromal cells under B lymphoid conditions in the presence of IL-7 for 2 days and then switched to myeloid culture conditions in the presence of M-CSF. (a) Flow cytometry of switched cultures (day 12) examining expression of CD19, Mac-1, F4/80 and CSF1R. (b) Cellular morphology of Mac-1+ cells generated under the switched culture conditions. Pax5-/- or Ikzf1-/- cells were sorted on the basis of Mac-1 expression and examined by Wright staining. Ikzf1-/- pro-B cells cultured on OP9 in the presence of IL-7 were used to compare morphology. All data are representative of three independent experiments.