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. Author manuscript; available in PMC: 2009 Jun 22.
Published in final edited form as: FEBS Lett. 2007 Jul 23;581(22):4153–4158. doi: 10.1016/j.febslet.2007.07.021

Figure 3. MSK1 enhances endogenous utrophin expression upon heregulin stimulation.

Figure 3

C2C12 cells transfected with either empty vector, pMT or MSK1 construct (pMSK1) were serum starved overnight followed by treatment with 2nM heregulin (HRG) for 30 minutes. MSK1 overexpression showed potentiation of heregulin-mediated utrophin (A) mRNA upregulation (Bargraph: pMT 0.9361±.040; pMT+HRG 1.503±.085; pMSK1 1.018±0.052; pMSK1+HRG 2.168±0.074; n=4). In contrast, heregulin did not show any effect on myostatin (B) mRNA expression (Bargraph: pMT 1.044±0.0.085; pMT+HRG 0.9979±0.139; pMSK1 1.139±0.225; pMSK1+HRG 1.035±0.034; n=4). The effect of MSK1 overexpression on heregulin-mediated utrophin upregulation was translated to protein level as revealed by anti-utrophin western blot (C). Tubulin was used as loading control. The band intensities of Western blot experiment were quantified and the ratio of utrophin and corresponding tubulin bands were plotted (D). ** statistical significance p<.05