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. 2009 Jun 17;2009:451084. doi: 10.1155/2009/451084

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Copyright © 2009 Michael Roth et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hsp40/70 rescue cells from heat stress. (a) Cells were pre-incubated with 5 or 10 nM of Hsp40 siRNA and then exposed to increasing heat stress (39–42°C) for 20 minutes. Following cells were kept under normal growth conditions for 48 hours and viable cells were counted after trypan blue exclusion staining. (b) Cells were pre-incubated with 5 or 10 nM of Hsp70 siRNA and treated as described above “a”. Data points represent the mean ± S.E.M. of triplicate experiments. ∗ indicates P < .01 compared to control (student's paired t-test, two-tailed). (c) Expression of caspase-3 by PDMM treated for various time intervals with increasing temperatures. Data points represent the mean ± S.E.M. of triplicate experiments.

Hsp40/70 rescue cells from heat stress. (a) Cells were pre-incubated with 5 or 10 nM of Hsp40 siRNA and then exposed to increasing heat stress (39–42°C) for 20 minutes. Following cells were kept under normal growth conditions for 48 hours and viable cells were counted after trypan blue exclusion staining. (b) Cells were pre-incubated with 5 or 10 nM of Hsp70 siRNA and treated as described above “a”. Data points represent the mean ± S.E.M. of triplicate experiments. ∗ indicates P < .01 compared to control (student's paired t-test, two-tailed). (c) Expression of caspase-3 by PDMM treated for various time intervals with increasing temperatures. Data points represent the mean ± S.E.M. of triplicate experiments.