Fig. 5.

PolyI:C leads to a reduction in uptake of soluble antigen by splenic DC. CD11c⁺ splenocytes were isolated by magnetic cell sorting. (A) The different DC subsets were identified by their differential expression of CD4, CD8, B220 and PDCA-1 as depicted. Surface expression of SR-A type I/II (CD204) in the different splenic DC subsets was analysed by flow cytometry. Grey filled and black histograms correspond to immunostaining with an isotype control and anti-CD204 antibody, respectively. (B) Histograms showing the uptake of OVA–Alexa555 by the different splenic DC subsets. Grey filled and black histograms correspond to DC cultured in the absence and presence of OVA–Alexa555, respectively. PolyI:C was used at a final concentration of 50 μg ml⁻¹. (C) Relative percentage of OVA–Alexa555⁺ CD8α⁺ DC in relation to CD8α⁺ DC incubated with the antigen in the absence of TLR agonists. PolyI:C, polyI, polyG and polyU were used at 50 μg ml⁻¹ while CpG was added to final concentration of 0.5 μg ml⁻¹. The figure compiles data from five independent experiments with the standard deviation depicted. For statistical analysis, Dunnett's multiple comparison test was used with the unstimulated cells serving as control group (*P < 0.05, **P < 0.01).