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. 2000 Jul 11;97(15):8606–8611. doi: 10.1073/pnas.150241097

Figure 1.

Figure 1

Coimmunoprecipitation of A1R and D1R. Cell membranes from A1/D1, A1/D2, or D1 cells were obtained and processed for immunoprecipitation (see Methods) by using the purified anti-A1R antibody PC11, the anti-D2R antibody, or an irrelevant goat IgG; all were covalently coupled to protein A-Sepharose. Immunoblottings of cell lysates (positive control) and immunoprecipitates were performed to detect A1R with anti-A1R antibody, D1R with anti-D1R antibody, or D2R with anti-D2R antibody. When indicated, A1/D1-cotransfected cells were incubated for 1 h with 10 μM SKF-38393 in the absence or presence of 100 nM R-PIA. The arrow indicates the band for A1R, the arrowhead the band corresponding to D1R, and the asterisk the band for D2R.