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. 2000 Jul 11;97(15):8606–8611. doi: 10.1073/pnas.150241097

Figure 4.

Figure 4

Distribution of A1R and D2R in A1/D2-cotransfected fibroblast cells. Cells were processed for immunostaining (see Methods) by using fluorescein (green)-conjugated rabbit anti-A1R antibody and a Texas red-conjugated rabbit anti-D2R antibody. The cells were analyzed by confocal laser microscopy. (B) Cells were treated with 100 nM R-PIA. Superimposition of images reveals the lack colocalization of A1R and D2R. (Scale bars: 10 μm.)