Figure 4.
Distribution of A1R and D2R in A1/D2-cotransfected fibroblast cells. Cells were processed for immunostaining (see Methods) by using fluorescein (green)-conjugated rabbit anti-A1R antibody and a Texas red-conjugated rabbit anti-D2R antibody. The cells were analyzed by confocal laser microscopy. (B) Cells were treated with 100 nM R-PIA. Superimposition of images reveals the lack colocalization of A1R and D2R. (Scale bars: 10 μm.)