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Mapping TSSs using primer extension and 5′-RACE. (A) Examples of primer extension. Arrowheads indicate runoff transcripts. (B) Examples of 5′-RACE. Each panel is a nondenaturing polyacrylamide gel photographed using Chemi Doc™ System (Bio-Rad). Lanes: M, 25 bp DNA ladder (Promega); +, RT–PCR products from TAP-treated RNA; –, RT–PCR products from untreated RNA (control). Solid arrowheads indicate TSSs. Open arrowheads indicate processing sites.
