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. 2009 May 13;37(11):e81. doi: 10.1093/nar/gkp273

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Preservation of histone PTMs by the acid-SP method. (A) Coomassie-stained 12.5% SDS–PAGE gel of samples obtained by SP after H₂SO₄ protein extraction from whole G7 and 1470.2 cells. Lane 1, input (I) H₂SO₄-crude extracted histone; lane 2, SP-FT fraction; lanes 3–5, 0.6 M NaCl wash; lane 6, core histones eluted at 2 M NaCl (E). (B) Immunoblotting of SP-input (I) and eluted (E) core histones. The antibodies used are indicated on the right of the blots. Bottom panel is a Ponceau S-stained nitrocellulose membrane of transferred histones.