Figure 6. Binding of PK 11195 to bacterial TSPO of Pseudomonas fluorescens MF37.

(a) Demonstration of the binding of [³H] PK 11195 at the position of the TSPO-immunoreactive band visualized by western blots in P. fluorescens MF37 extracts. (b) Scatchard plot showing the binding characteristics of PK 11195 to TSPO of P. fluorescens MF37. The maximal binding potential (Bmax) is 0.087 pmole.µ g⁻¹ bacterial protein and the Kd is 0.92 nM.