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. Author manuscript; available in PMC: 2009 Jun 22.
Published in final edited form as: Dev Biol. 2007 Oct 24;313(1):234–245. doi: 10.1016/j.ydbio.2007.10.014

Figure 6. Kinetics of CreERT2 sub-cellular localization following tamoxifen injection.

Figure 6

A–D, CreERT2 localization using anti-Cre antibody staining in E16.5 kidneys; A′–D′ corresponding images with DAPI overlay to illustrate nuclei. Kidneys from uninjected animals (A, A′) show cytoplasmic localization of Cre (arrows). 8 hours after tamoxifen injection (B, B′) Cre is seen in cytoplasmic (arrows) and nuclear compartments (arrowheads). 24 hours after injection (C, C′) Cre is found almost exclusively in nuclei (arrowheads). 96 hours post-injection (D, D′) Cre has redistributed to the cytoplasm in the cap mesenchyme (arrows). UB-ureteric bud.