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. Author manuscript; available in PMC: 2009 Jun 22.
Published in final edited form as: Nat Methods. 2008 Jan 20;5(2):171–173. doi: 10.1038/nmeth.1177

Figure 2. Gluc reporter in the blood as a useful tool to monitor in vivo processes.

Figure 2

(a and b) 1×105 Gli36-Gluc cells were implanted in the brains of nude mice (n=3/group) and tumor growth was monitored by in vivo bioluminescence imaging (a) or by measuring Gluc activity using the luminometer (b). (c and d) 1×106 Gli36 cells were implanted subcutaneously and tumors were either injected with LV-Gluc-CFP or PBS, 3 days later. Viral delivery was monitored over time by measuring Gluc activity in blood samples (c), by in vivo bioluminescence imaging using the CCD camera (d, upper panel) and by monitoring CFP expression in tumor sections (d, lower panel). (e and f) One millions C17.2 NPCs expressing Gluc and CFP (e, upper panel) or PBS were injected i.v. in nude mice. Gluc activity was monitored over time using the CCD camera (e, lower panel) and in blood samples using the luminometer (f). Data shown are from a representative mouse from each set. Scale bar, 100 μm.