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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2009 May 3;238(1):64–70. doi: 10.1016/j.taap.2009.04.015

Figure 1. Activated microglia contribute to astrocyte proliferation.

Figure 1

(A) Primary enriched astrocyte cultures with or without microglia within insert wells were treated with various concentrations of LPS in a final volume of 200 μl/well for 72 h. (B) Primary enriched microglial cultures were treated with different concentrations of LPS for 72 h, then the conditioned media was collected and added to primary enriched astrocyte cultures. Incubation was lasted for an additional 72 h. Cell proliferation was assayed using a BrdU ELISA kit as described in Material and Methods. Results are mean ± S.E.M of three experiments performed in triplicate. * p <0.05, compared with corresponding control cultures; # p <0.05, compared with astrocytes alone after same treatment.