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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2009 May 3;238(1):64–70. doi: 10.1016/j.taap.2009.04.015

Figure 2. LPS treatment induced COX-2 gene and protein expression in primary enriched microglial and astrocyte cultures.

Figure 2

Enriched microglial and astrocyte cultures were treated with different concentrations of LPS. Then COX-2 gene expression was assayed using RT-PCR (A) at 6 h after treatment and COX-2 protein expression was assayed using Western blot (B) at 24 h post treatment. Quantitative analysis of COX-2 protein expression was shown in C. Data have been normalized to GAPDH and are mean ± S.E.M of three experiments performed in triplicate. * p <0.05, compared with corresponding control cultures; # p <0.05, compared with astrocytes after same treatment.