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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2009 May 3;238(1):64–70. doi: 10.1016/j.taap.2009.04.015

Figure 5. Microglia from COX-2−/− mice showed impaired effect in mediating astrocyte activation.

Figure 5

Primary enriched astrocyte cultures from COX-2+/+ mice along with insert wells with microglia from COX-2+/+ and COX-2−/− mice were treated with different concentrations of LPS for 72 h. (A) Cell proliferation was assayed using a BrdU ELISA kit. (B) The supernatant were collected 72 h after treatment and PGE2 concentration was assayed using an EIA kit. Results are mean ± S.E.M of three experiments performed in triplicate. * p <0.05, compared with corresponding control cultures; # p <0.05, compared with insert wells plus COX-2+/+ microglial cultures.